Mario is the titular main protagonist of the Super Mario series and the sole playable character in Super Mario 64. He is a hero from the Mushroom Kingdom who saves Princess Peach from the clutches of the evil king of the Koopas, Bowser.
- Before Charles Martinet's voice clips were implemented into the game, Mario used to use samples as a placeholder. These samples are from Best Service Voice Spectral and Warner Brothers' sound effects library.
- Mario's voice samples from this game would go reused in Super Smash Bros. Melee and Super Mario Sunshine.
- In Super Smash Bros. Melee, all of Mario's voice lines would originate from Super Mario 64 with the exception of one, which has Mario going "yeehaw". The existence of this voice clip amongst every other voice clip originating from Super Mario 64 implies that this voice clip could've been originally slated to appear in Super Mario 64, but was cut due to a lack of use.